Generated:Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
Article Snippet: .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650). .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650).
Expressing:Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
Article Snippet: .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650). .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650).
Plasmid Preparation:Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
Article Snippet: .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650). .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650).
Polymerase Chain Reaction:Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
Article Snippet: .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650). .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650).
Clone Assay:Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
Article Snippet: .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650). .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650).
Cloning:Article Title: The NLRP6 inflammasome is activated by sterile or pathogen-induced endolysosomal damage.
Article Snippet: .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650). .. ASC-mCherry was generated using an expression plasmid for ASCGFP (Sino Biological #HG11175-ANG) as PCR template (PrimeSTAR MAX DNA Polymerase, Takara Bio #R045A) and was cloned into pLJM1_EGFP (addgene #19319) backbone, using AgeI-BstBI (New England Biolabs #R3552L and #R0519L) restriction digest and In-Fusion cloning (Takara Bio #6396650).
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